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Laboratory for Langerhans Cell Research


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Dendritic cells are key regulators in the immune system. They are leukocytes that occur in low numbers in most tissues and organs of the body. Their main function is antigen presentation to T lymphocytes, hence in one side they can generate immunity and on the other side maintain peripheral tolerance to self antigens.


Our working group is interested in deciphering in more detail the role of skin dendritic cells in the skin immune system with special focus on tumor immunity. In an attempt to answer this question we investigate the migration and antigen presentation by skin dendritic cells that is required for the induction of immune responses. The understanding of the role of cutaneous dendritic cells in the skin immune system will allow us to design novel immunotherapies against skin cancer and infections.



Dendritic cells as antigen presenting cells

     Dendritic cells (DC) are bone marrow derived cells that develop alongside monocytes and macrophages. Their unique ability to stimulate naive T cells explains why they are called „nature´s adjuvant“. DC reside in lymphatic and peripheral tissues, like spleen, lymph node, skin, lung and the gut. In these locations DC fullfill different tasks such as antigen capture in the periphery and antigen presentation to T cells in the lymphoid organs. During their life cycle immature DC survey peripheral tissue for invading pathogens and tumor development. Immature DC are very efficient in phagocytosis and macropinocytosis, and after antigen incorporation they start their maturation program. Inside the cells the antigenic proteins are processed into small peptides, which can bind to MHC (major histo-compatibility complex)-class I and II molecules. DC migrate from peripheral tissue through lymphatic vessels to the draining lymph nodes. Upon arrival DC present these MHC/peptide complexes on the cell surface to CD4+ and CD8+ T cells. As a consequence, T cells start to proliferate and differentiate into effector cells, which can secrete cytokines and kill infected and tumor cells.


 Dendritic cells in control of immunity and tolerance

      DC are key regulators of immunity given the fact that they are required for induction of immune responses in infectious diseases, allergy and cancer. The main function of DC is the activation of adaptive immunity, yet, DC can also interact with innate immune cells, for instance natural killer (NK) and NKT cells. Hence, DC function as a bridge between innate and adaptive immunity. DC are not merely limited to pathogen recognition, but are involved in immune responses against allergens and self-antigens,, thus, they contribute to autoimmunity and cancer. They are crucial for the initiation and maintenance of central and peripheral tolerance and regulate the balance between immunity and tolerance. Persistence of tumors is commonly caused by induction of tolerance against tumor specific antigens leading to immune evasion of the tumor. In fact, DC are promising targets for immuno-therapy and are currently tested in clinical trials for treatment of cancer and autoimmunity.


 Cutaneous dendritic cells

     The skin consists of two compartments, the epidermis and the dermis, each populated by different subsets of DC. The Langerhans cells (LC) reside in the suprabasal layers of the epidermis surrounded by keratinocytes, the epithelial cells building up this compartment. In the dermis DC are distributed throughout the connective tissue and very recently, these dermal DC were subdivided into two subsets. The function and relevance of the diverse cutaneous populations is under dispute and there is some indication that dermal DC and LC might play different roles in the induction of immune responses in skin-draining lymph nodes. Dermal DC and LC can be generated from CD34+ precursors and these subset induce different immune responses when tested in vitro. LC and dermal DC can stimulate cytotoxic T lymphocytes responses, however LC proved to be more potent than dermal DC. Moreover, it was postulated that dermal DC might be involved in humoral immune responses, since they are able to differentiate B cells to antibody secreting plasma cells and they are localized close to the B cell area in the lymph node. Current dogma has it that LC and dermal DC fulfil the role of the first defence line against microbes invading the body through the skin, however, this concept has recently been challanged for certain viral and parasitic infections.


 Dendritic cells as targets for immunotherapy

     DC as the most potent inducers of primary T cell responses are promising candidates for immunotherapy against cancer. Clinical trials in which DC have been used as carriers for tumor antigens have met with some success. In conventional DC immunotherapies, blood monocytes or CD34+ precursor cells from patients are differentiated into DC in vitro, before they are loaded with tumor antigen and injected back into cancer patients. Some cancer patients with solid malignancies, such as renal cell carcinoma and melanoma showed partial clinical responses or even complete remissions. As this treatment is cumbersome and expensive, we have started to investigate simpler and cheaper ways for immunotherapy. Most importantly, however, LC are capable of stimulating potent T cell responses which makes them optimal targets for immunization strategies through the skin.

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Research Topics

immune function of dendritic cells

role of skin dendritic cells (e.g. Langerhans cells) in tumor immunity

Development of novel immunotherapeutical strategies against skin cancer

A recent (11/2015) video report by the Medical University of Innsbruck about our lab - unfortunately, only in German....

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some lab pictures


Patrizia Stoitzner, Ph.D., Professor

 Head of the Langerhans Cell Research Lab. Patrizia studied microbiology with focus on biochemistry at the University of Innsbruck. During her master and Ph.D. thesis performed at the Department of Dermatology (Medical University of Innsbruck), she worked on the migration of skin DC and identified the routes and regulation of this process. She was involved in the characterization of Langerin as a novel marker for Langerhans cells (LC) which allowed for the first time to track LC during their migration to the lymph node and investigate their function. After finishing her Ph.D. in 2001 she investigated the ability of LC to present protein antigen to CD4+ and CD8+T cells and started to develop a novel immunization strategy through the skin, the epicutaneous immunization. This approach takes advantage of the potency of skin DC in inducing T cell responses by loading resident skin DC in situ with protein antigen for antigen presentation. During a post doctoral fellowship at the Malaghan Insitute of Medical Research in Wellington, New Zealand, she tested this novel immunization strategy in mouse tumor models and proved its effectiveness as a potential new treatment against skin cancer. Since her return in 2007 she has established her own research lab with a research focus on the role of skin DC in tumor immunity. With spontaneous and inducible skin cancer models she wants to investigate the functional role of skin DC in tumor immunity and how this knowledge can be translated into novel immunotherapeutical strategies. As of April 2020 Patrizia is Full Professor for Experimental Dermatology with specialization in tumor immunology ("Professorin für Experimentelle Dermatologie mit dem Schwerpunkt  Tumorimmunologie"). Bibliography


Christoph H. Tripp, Ph.D.

Senior Scientist. Christoph studied microbiology at the University of Innsbruck. His undergraduate thesis on the ontogeny of Langerin expression in the epidermis of mice was performed at the Department of Dermatology, Medical University of Innsbruck. During his Ph.D. time he investigated the effects of toll-like receptor ligand application to the skin in a mouse model of DC vaccination - one approach to enhance migration of injected DC and subsequent T cell responses. During his post doctoral fellowship he established a mouse model for squamous cell carcinoma that is induced by carcinogens applied to the skin. Currently, Christoph is working on the characterization of immune infiltrates in patient samples of various skin cancer entities with special focus on DC subsets. He is the head of our animal facility and our specialist for flow cytometry and cell sorting. Bibliography


Daniela Ortner-Tobider, Ph.D.

Senior post doctoral fellow and recent (2014) awardee of a Hertha Firnberg career development grant for young female scientists of the Austrian Science Fund (FWF). Daniela studied microbiology and molecular biology at the University of Innsbruck. She completed her undergraduate thesis in the laboratory of Prof. Cornelia Lass-Flörl at the Department of Hygiene and Microbiology (Medical University of Innsbruck) in 2005, investigating the interaction of Aspergillus spp. and platelets. During her Ph.D. thesis, which she performed in the laboratory of Assoc.-Prof. Christine Heufler-Tiefenthaler at the Department of Dermatology (Medical University of Innsbruck), she investigated differentially expressed molecules during the maturation of human DC, in particular the molecular and functional characterization of the adaptor molecule Bam32. After her Ph.D. she joined the lab of Prof. D.H. Kaplan in Minneapolis, MN, US to study skin DC subsets and their functional properties in various mouse models. She has restarted her scientific career after a year working in industry (Sandoz-Novartis Austria) and a year of maternal leave. Since she has joined our lab in 2012 she has been working on a chemically induced mouse model for squamous cell carcinoma and investigates the immunological features of this tumor entity. Her focus is on understanding the specific role of the various skin DC subsets in immunological responses to non-melanoma skin cancer. Bibliography


Claudia Zelle-Rieser, PhD, Senior Scientist

Claudia joined the lab in September 2018. She will contribute with her long-standing expertise in dendritic cell biology.


Helen Strandt, PhD, postdoctoral fellow

Helen started working in the lab in 2021. One of the topics of her interest is the pathogenesis of vitiligo and the involvement of the NLRP3 inflammasome in this skin disease. She recently acquired FWF funding for this project.


Sophie Dieckmann, MSc, PhD student

Sophie started her PhD thesis in the lab in 2021.


Nikolaus Romani, Ph.D., Professor emeritus

Head of the Experimental Dermatology Unit. Niki studied biology at the University of Innsbruck where he obtained his Ph.D. in 1983. After two years at the Rockefeller University in Ralph Steinman's laboratory (1987-88) he - together with Gerold Schuler - helped to make Innsbruck one of the first "dendritic cell (DC) strongholds" in the Old World. Since then he has pursued DC research with focus on investigating their functional properties to harness these cells for DC-based immunotherapy at the Department of Dermatology, Venereology & Allergology of the Medical University of Innsbruck. He became Full Professor for Experimental Dermatology in 2012. He emerited in October 2018. Bibliography


Former lab members:

Athanasios (Thanasis) Seretis, M.Sc.

PhD student. Thanasis studied Biology at the Aristotle University of Thessaloniki and continued his Master studies on Molecular Biology and Biomedicine at the University of Crete, where he graduated in September of 2014. He continued his research at the lab of Autoimmunity and Inflammation as a research assistant, until the summer of 2016. Thanos will perform his PhD thesis within the framework of the EU training network IMMUTRAIN where Prof. Stoitzner is heading a subproject that deals with the combination of antibodies with dendritic cell therapy for cancer. Thanasis successfully completed his PhD thesis, entitled "Combination Therapies to Improve Dendritic Cell-based Treatment of Melanoma", in April 2021.


Lydia Bellmann, Ph.D.

Lydia studied Molecular Medicine at the University of Erlangen-Nuremberg in Germany, where she obtained her Bachelor’s degree. After her bachelor studies, she did a research internship at Roche in Penzberg, Germany and at the Trinity Biomedical Sciences Institute in Dublin, Ireland. For her master degree, she studied Molecular Cell and Developmental Biology at the Leopold-Franzens-University, Innsbruck and completed her master thesis on immunosurveillance in different melanoma mouse models in our lab. Since August 2016, Lydia was a PhD student in our lab where she continues working on her master thesis project and on antibody-mediated targeting for immunotherapeutical approaches in human skin. She successfully completed her PhD studies with a thesis entitled "Targeting antigens to C-type lectin receptors on human skin dendritic cells to enhance immune responses against cancer" and graduated in July 2020. Bibliography


Anastasia (Natasa) Prokopi, M.Sc.

Ph.D. student. Natasa studied Biology at the University of Patras in Greece, where she obtained her Bachelor’s degree. She completed her master thesis in the Laboratory of Prof. Dr. Ferry Ossendorp at the Leiden University Medical Center in the Netherlands, where she worked on cross-presentation of tumour antigens after in vitro Photodynamic Therapy. Currently she is working on her Ph.D. thesis about the functional role of dendritic cells in melanoma with a special focus on their interaction with T cells and myeloid-derived suppressor cells. She is a member of the PhD program (doctoral college) on Molecular Cell Biology and Oncology (MCBO), financed by the Austrian Science Fund (FWF). She successfully completed her PhD with the thesis entitled "The duality of the immune system in melanoma" in January 2020.


Kerstin Komenda, B.Sc.

Biomedical technician. She has joined our lab in 2013 and her main focus is on genotyping mice and preparing tissue for flow cytometric analysis. In addition, she assists with testing novel therapeutical options in mouse models for skin cancer. She has also become an expert in isolating dendritic cells from human skin.


Rita Steindl, B.Sc.

Rita began her master studies in early 2019, and compelted them successfully in early 2020.


Giuseppe Cappellano (PhD, Senior Post-Doc)

Giuseppe started working in our lab in July 2017 as a senior postdoctoral fellow. He contributed significantly to several projects with his knowledge and profound expertise. Unfortunately, he had to leave us in September 2018. He has now a position as a professor at the Università degli Studi del Piemonte Orientale at Novara, Italy. Bibliography


Johanna Schachtl-Rieß, B.Sc.

Master student. Johanna successfully completed her master thesis in Molecular Medicine in March 2018. She continues with a PhD in the labs of Prof. Florian Kronenberg, Gentic Epidemiology. Good luck!


Katharina Hutter, M.Sc.

Katharina successfully completed her master studies in July 2017. She will move on to the lab of Prof. Villunger at the Biocenter / CCB (Division of Developmental Immunology) to do a PhD. We wish her luck!


Sandra Schaffenrath, PhD

Sandra studied microbiology with focus on medical microbiology at the University of Innsbruck. During a stay abroad at the Lund University, Sweden, she deepened her knowledge in the field of immunology and completed her master thesis in the laboratory of Assoc.-Prof. Cornelia Speth at the Department of Hygiene and Microbiology (Medical University of Innsbruck) by focusing on innate immunity in the context of invasive mycosis. Since March 2013 Sandra has been working as a Ph.D. student in our lab with special emphasis on harnessing DC for cancer immunotherapy by antigen targeting. She completed her PhD thesis successfully with distinction in October 2016. Bibliography


Hermann Voit, M.Sc.

Master student. Hermann was a student of biotechnology at the MCI Innsbruck. He successfully completed his Master in September 2016.


David Mairhofer, M.Sc., Ph.D.

Ph.D. student. David studied microbiology at the University of Innsbruck where he obtained his Bachelor degree. He completed his master thesis in the Laboratory of Pavel Kovarik at the Max F. Perutz Laboratories in Vienna, where he was working on the role of tristetraprolin in regulation of mRNA stability and skin immunity. He has been working on his Ph.D. thesis about the role of DC in skin cancer with a special emphasis on T cell responses against melanoma. He is a member of the doctoral college on molecular cell biology and oncology (MCBO), financed by the Austrian Science Fund (FWF). He passed the defense of his thesis with distinction and is now (as of October 12th 2015)Ph.D.! Bibliography


David Schlögl, M.Sc.

Diploma student (of Leopold-Franzens-University, Innsbruck). He investigated the immune infiltrates in a chemically induced mouse model for squamous cell carcinoma and a spontaneous melanoma mouse model by immunofluorescence microscopy. Furthermore, he contributed seminally to our description of Langerhans cells in the sebaceous glands of mouse skin. He completed is diploma studies successfully in July 2015. Bibliography


Florian Sparber, Ph.D.

Ph.D. student and post doctoral fellow. Florian studied molecular biology at the University of Innsbruck. He completed his undergraduate thesis in our lab in 2008, investigating the incorporation of protein antigens by murine LC in situ and in vivo. During his Ph.D. time he worked in collaboration with the Biocenter Innsbruck on the role of the adaptor protein p14, a member of the extracellular signal regulated kinase (ERK) cascade in DC, as an attempt to link signal transduction with the functional aspects of DC. He finished his Ph.D. with honors in 2013 and has left the lab in February 2014 for a post doctoral fellowship in the lab of Prof. Salomé LeibundGut-Landmann, first at the ETH Zürich and since mid-2015 at the University of Zürich. Bibliography


Vincent Flacher, Ph.D.

Post doctoral fellow. Vincent worked as a post doctoral fellow in our lab on immuntherapeutical strategies to target skin DC in situ. He used antibody-antigen complexes for intradermal vaccinations in mouse models. He left our lab in 2013 to take a position at CNRS, Institut de Biologie Moleculaire et Cellulaire in Strasbourg. Bibliography


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Selected publications (as of May 2022)

Stoitzner P, Romani N, Rademacher C, Probst HC, Mahnke K. Antigen targeting to dendritic cells: still a place in future immunotherapy? Eur J Immunol, in press, 2022 (Review article)

Bellmann L, Strandt H, Zelle-Rieser C, Ortner D, Tripp CH, Schmid S, Rühl J, Cappellano G, Schaffenrath S, Prokopi A, Spoeck S, Seretis A, Del Frari B, Sigl S, Krapf J, Heufler C, Keler T, Münz C, Romani N, Stoitzner P. Targeted delivery of a vaccine protein to Langerhans cells in the human skin via the C-type lectin receptor Langerin. Eur J Immunol, in press, 2022. free article

Prokopi A, Tripp CH, Tummers B, Hornsteiner F, Spoeck S, Crawford JC, Clements DR, Efremova M, Hutter H, Bellmann L, Cappellano G, Cadilha BL, Kobold S, Boon L, Ortner D,  Trajanoski Z, Chen S, de Gruijl TD, Idoyaga J, Green DR, Stoitzner P. Skin dendritic cells in melanoma are key for successful checkpoint blockade therapy. Journal for Immunotherapy of Cancer. 9:e000832. doi:10.1136/jitc-2020-000832. 2021. free article

Tripp CH, Voit H, An A, Seidl-Philipp M, Krapf J, Sigl S, Romani N, Del Frari B, Stoitzner P. Laser-assisted epicutaneous immunization to target human skin dendritic cells. Experimental Dermatology. 30:1279–1289, 2021. free article

Bellmann L, Zelle-Rieser C, Milne P, Resteu A, Tripp CH, Hermann-Kleiter N, Zaderer V, Wilflingseder D, Hörtnagl P, Theochari M, Schulze J, Rentzsch M, Del Frari B, Collin M, Rademacher C, Romani N, Stoitzner P. Notch-mediated generation of monocyte-derived Langerhans cells: Phenotype and function. J Invest Dermatol, 141:84-94, 2021. free article

Eisendle K, Weinlich G, Ebner S, Forstner M, Reider D, Zelle-Rieser C, Tripp CH, Fritsch P, Stoitzner P, Romani N, Nguyen VA. Combining chemotherapy and autologous peptide-pulsed dendritic cells provides survival benefit in stage IV melanoma patients. J Dtsch Dermatol Ges. JDDG. 18:1270-1277, 2020. free article

Eichin D, Pessia A, Takeda A, Laakkonen J, Bellmann L, Kankainen M, Imhof BA, Stoitzner P, Tang J, Salmi M, Jalkanen S. CD73 contributes to anti-inflammatory properties of afferent lymphatic endothelial cells in humans and mice. Eur J Immunol. 2020, doi: 10.1002/eji.201948432. free article

Bellmann L, Cappellano G, Schachtl-Riess JF, Prokopi A, Seretis A, Ortner D, Tripp CH, Brinckerhoff CE, Mullins DW,  Stoitzner P. A TLR7 agonist strengthens T and NK cell function during BRAF-targeted therapy in a preclinical melanoma model. Int J Cancer. 146:1409-1420, 2020. free article

Peiffer L, Farahpour F, Sriram A, Spassova I, Hoffmann D, Kubat L, Stoitzner P, Gambichler T, Sucker A, Ugurel S, Schadendorf D, Becker JC. BRAF and MEK inhibition in melanoma patients enables reprogramming of tumor infiltrating lymphocytes. Cancer Immunol Immunother. doi: 10.1007/s00262-020-02804-4. in press 2020. free article

Grund-Gröschke S, Ortner D, Szenes-Nagy AB, Zaborsky N, Weiss R, Neureiter D, Wipplinger M, Risch A, Hammerl P, Greil R, Sibilia M, Gratz IK, Stoitzner P, Aberger F. Epidermal activation of Hedgehog signaling establishes an immunosuppressive microenvironment in basal cell carcinoma by modulating skin immunity. Mol Oncol. 14:1930-1946, 2020. free article

Clausen BE, Romani N, Stoitzner P. Meeting Report of the 16th International Langerhans Cell Workshop: Recent Developments in Langerhans Cell and Skin Dendritic Cell Biology and their Therapeutic Application. J Invest Dermatol. 140:1315-1319, 2020

Schulze J, Rentzsch M, Kim D, Bellmann L, Stoitzner P, Rademacher C. A liposomal delivery platform for protein antigen delivery to Langerin-expressing cells. Biochemistry. 58:2576-2580, 2019. free article

Wamhoff E-C, Schulze J, Bellmann L, Rentzsch M, Bachem G, Fuchsberger FF, Rademacher J, Hermann M, Del Frari B, van Dalen R, Hartmann D, van Sorge NM, Seitz O, Stoitzner P, Rademacher C. A specific, glycomimetic Langerin ligand for human Langerhans cell targeting. ACS Central Science, 5:808-820, 2019. free article

Koske I, Rössler A, Pipperger L, Petersson M, Barnstorf I, Kimpel J, Tripp CH, Stoitzner P, Bánki Z, von Laer D. Oncolytic virotherapy enhances the efficacy of a cancer vaccine by modulating the tumor microenvironment. Int J Cancer. 145:1958-1969, 2019. free article

Sparber F, Dolowschiak T, Mertens S, Lauener L, Clausen BE, Joller N, Stoitzner P, Tussiwand R, LeibundGut-Landmann S. Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection. PLoS Pathog. 14(5):e1007069. May 2018. free article

Ober-Blöbaum JL, Ortner D, Haid B, Brand A, Tripp C, Clausen BE, Stoitzner P.Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry. Methods Mol Biol, 1559:37-52, 2017

Ortner D, Tripp CH, Komenda K, Dubrac S, Zelger B, Hermann M, Doppler D,  Tymoszuk PZ, Boon L, Clausen BE, Stoitzner P. Langerhans cells and NK cells cooperate in the inhibition of chemical skin carcinogenesis. Oncoimmunology, 6(2):e1260215, 2016. free article

Clausen BE, Stoitzner P. Functional specialization of skin dendritic cell subsets in regulating T cell responses. Front Immunol, 6:534, 2015. free article

Mairhofer DG, Ortner D, Tripp CH, Schaffenrath S, Fleming V, Heger L, Komenda K, Reider D, Dudziak D, Chen S, Becker JC, Flacher V, Stoitzner P. Impaired gp100-specific CD8+ T cell responses in the presence of myeloid-derived suppressor cells in a spontaneous mouse melanoma model. J Invest Dermatol, 135:2785-2793, 2015. free article

Haid B, Schlögl D, Hermann M, Tripp CH, Stoitzner P, Romani N, Flacher V. Langerhans cells in the sebaceous glands of the murine skin. Exp Dermatol. 2015 Jul 14. doi: 10.1111/exd.12803. [Epub ahead of print] free article

Sparber F, Tripp CH, Komenda K, Scheffler JM, Clausen BE, Huber LA, Romani N, Stoitzner P. The late endosomal adaptor molecule p14 (LAMTOR2) regulates TGFβ1-mediated homeostasis of Langerhans cells. J Invest Dermatol, 135:119-129, 2015. free PMC article

Stoitzner P, Schaffenrath S, Tripp CH, Reider D, Komenda K, Del Frari B, Djedovic G, Ebner S, Romani N. Human skin dendritic cells can be targeted in situ by intradermal injection of antibodies against lectin receptors. Exp Dermatol, 23:909-915, 2014. free article
Flacher V, Tripp CH, Mairhofer D, Steinman RM, Stoitzner P, Idoyaga J, Romani N. Antigen capture via CD207 molecules on skin dendritic cells can either prime or tolerize CD8+T cells. EMBO Mol Med, 6:1191-1204, 2014 free article
Scheffler JM, Sparber F, Herrmann C, Blitz J, Romani N, Stoitzner P, Huber LA. LAMTOR2 regulates dendritic cell homeostasis through FLT3 dependent mTOR signaling. Nature Communications, 5:5138, 2014 free article
Sparber F, Scheffler J, Amberg N, Tripp CH, Heib V, Hermann M, Zahner SP, Clausen BE, Reizis B, Huber LA, Stoitzner P, Romani N. The late endosomal adaptor molecule p14 (LAMTOR2) represents a novel regulator of Langerhans cell homeostasis. Blood, 123:217-227, 2013. free article
Romani N, Tripp CH, Stoitzner P. Langerhans cells come in waves. Immunity, 37:766-768, 2012. Free article
Flacher V, Tripp CH, Haid B, Kissenpfennig A, Malissen B, Stoitzner P, Idoyaga J, Romani N. Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses. J Immunol, 188:2146-2155, 2012 free article
Romani N, Flacher V, Tripp CH, Sparber F, Ebner S, Stoitzner P. Targeting skin dendritic cells to improve intradermal vaccination. Current Topics in Microbiology & Immunology, 351:113-138, 2012
Schwingshackl P, Obermoser G, Nguyen VA, Fritsch P, Sepp N, Romani N. Distribution and Maturation of Skin Dendritic Cell Subsets in Two Forms of Cutaneous T Cell Lymphoma - Mycosis Fungoides and Sézary Syndrome. Acta Derm Venereol, 92:269-275, 2012 free article
Romani N, Brunner P, Stingl G. Changing views of the role of Langerhans cells. J Invest Dermatol, 132:872-881, 2012 free article
Stoitzner P, Romani N. Langerin, the "Catcher in the Rye": An important receptor for pathogens on Langerhans cells. Commentary. Eur J Immunol, 41:2526-2529, 2011
Nguyen VA, Dubrac S, Huter O, Del Frari B, Romani N, Ebner S. CD34+ derived Langerhans cell-like cells are different from epidermal Langerhans cells in their response to thymic stromal lymphopoietin. J Cell Mol Med, 15:1847-1856, 2011
Noordegraaf M, Flacher V, Stoitzner P, Clausen BE. Functional redundancy of Langerhans cells and Langerin+dermal dendritic cells in contact hypersensitivity. J Invest Dermatol, 130:2752-2759, 2010 free article
Sparber F, Tripp CH, Hermann M, Romani N, Stoitzner P. Langerhans cells and dermal dendritic cells capture protein antigens in the skin: possible targets for vaccination through the skin. Immunobiology. 215(9-10):770-9, 2010 free article
Stoitzner P. The Langerhans cell controvery: are they immunostimulatory or imunoregulatory cells of the skin immune system? Immunol. Cell Biol. 88: 348-350, 2010
Stoitzner P, Sparber F, Tripp CH. Langerhans cells as targets for immunotherapy against skin cancer. Immunol. Cell Biol. 88: 431-437, 2010 free review article
Romani N, Thurnher M, Idoyaga J, Steinman RM, Flacher V. Targeting of antigens to skin dendritic cells: possibilities to enhance vaccine efficacy. Immunol Cell Biol. 88: 424-430, 2010 free review article
Romani N, Clausen BE, Stoitzner P. Langerhans Cells & More: Langerin-expressing dendritic cell subsets in the skin. Immunol Rev, 234:120-141, 2010 (Review Article) free review article
Stoitzner P, Romani N, McLellan AD, Tripp CH, Ebner S. Isolation of Skin Dendritic Cells from Mouse and Man. Methods Mol Biol, 595:235-248, 2010 (Methods Article)
Romani N, Merad M, Stingl G, Stoitzner P. Langerhans cells at the interface of medicine, science, and industry. J Invest Dermatol, 130:331-335, 2010 (Free Meeting Report).
Tripp CH, Ebner S, Ratzinger G, Romani N, Stoitzner P. Conditioning of the Injection Site With CpG Enhances the Migration of Adoptively Transferred Dendritic Cells and Endogenous CD8+ T-cell Responses. J Immunother, 33:115-125, 2010
Flacher V, Tripp CH, Stoitzner P, Haid B, Ebner S, Del Frari B, Koch F, Park CG, Steinman RM, Idoyaga J, Romani N. Epidermal Langerhans cells rapidly capture and present antigens from c-type lectin-targeting antibodies deposited in the dermis. J Invest Dermatol, 130:755-762, 2010 free article
Tripp CH, Sparber F, Hermans IF, Romani N, Stoitzner P. Glycolipids injected into the skin are presented to NKT cells in the draining lymph node independently of migratory of migratory skin dendritic cells. J Immunol, 182:7644-54, 2009 free article
Flacher V, Sparber F, Tripp CH, Romani N, Stoitzner P. Targeting of epidermal Langerhans cells with antigenic proteins: attempts to harness their properties for immunotherapy. Cancer Immunol Immunother, 1137-47, 2009
Stoitzner P, Green LK, Jung JY, Price KM, Tripp CH, Malissen B, Kissenpfennig A, Hermans IF, Ronchese F. Tumor immunotherapy by epicutaneous immunization requires Langerhans cells. J Immunol, 180:1991-1998, 2008 free article
Stoitzner P, Green L, Jung JY, Price KM, Haley A, Kivell B, Ronchese F. Inefficient presentation of tumor-derived antigen by tumor-infiltrating dendritic cells. Cancer Immunol Immunother, 57:1665-73, 2008
Flacher V, Douillard P, Aït-Yahia S, Stoitzner P, Clair-Moninot V, Romani N, Saeland S. Expression of Langerin / CD207 reveals dendritic cell heterogeneity between inbred mouse strains. Immunology, 123:339-347, 2008
Tripp CH, Haid B, Flacher V, Sixt M, Peter H, Farkas J, Gschwentner R, Sorokin L, Romani N, Stoitzner P. The lymph vessel network in mouse skin visualised with antibodies against the hyaluronan receptor LYVE-1. Immunobiology, 213: 715-728, 2008
Stoitzner P, Tripp CH, Eberhart A, Price KM, Jung JY, Bursch L, Ronchese F, Romani N. Langerhans cells crosspresent antigen derived from skin. Proc Natl Acad Sci USA, 103:7783-7788, 2006 free article
Stoitzner P, Tripp CH, Douillard P, Saeland S, Romani N. Migratory Langerhans cells in mouse lymph nodes in steady state and inflammation. J Invest Dermatol, 125:116-125, 2005
Holzmann S, Tripp CH, Schmuth M, Janke K, Koch F, Saeland S, Stoitzner P, Romani N. A model system using tape stripping for characterization of Langerhans cell-precursors in vivo. J Invest Dermatol, 122:1165-1174, 2004
Stoitzner P, Holzmann S, McLellan AD, Ivarsson L, Stössel H, Kapp M, Kämmerer U, Douillard P, Kämpgen E, Koch F, Saeland S, Romani N. Visualization and characterization of migratory Langerhans cells in murine skin and lymph nodes by antibodies against Langerin / CD207. J Invest Dermatol, 120:266-274, 2003 free article
Ratzinger G, Stoitzner P, Ebner S, Lutz MB, Layton GT, Rainer C, Senior RM, Shipley MJ, Fritsch P, Schuler G, Romani N. Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. J Immunol, 168:4361-4371, 2002 free article
Koch F, Stanzl U, Jennewein P, Janke K, Heufler C, Kämpgen E, Romani N, Schuler G. High level Interleukin-12 production by murine dendritic cells: upregulation via MHC class II and CD40 molecules and downregulation by Interleukin-4 and Interleukin-10. J Exp Med, 184:741-746, 1996
Koch F, Trockenbacher B, Kämpgen E, Grauer O, Stössel H, Livingstone AM, Schuler G, Romani N. Antigen processing in populations of mature murine dendritic cells is caused by subsets of incompletely matured cells. J Immunol, 155:93-100, 1995
Romani N, Gruner S, Brang D, Kämpgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman RM, Schuler G. Proliferating dendritic cell progenitors in human blood. J Exp Med, 180:83-93, 1994
Romani N, Koide S, Crowley M, Witmer-Pack M, Livingstone AM, Fathman CG, Inaba K, Steinman RM: Presentation of exogenous protein antigens by dendritic cells to T cell clones: intact protein is presented best by immature epidermal Langerhans cells. J Exp Med 169: 1169-1178, 1989
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Laboratory for Research on Langerhans Cells
Department of Dermatology, Venereology & Allergology
Anichstrasse 35
6020 Innsbruck

Phone: +43-(0)512-504-DDI

Patrizia Stoitzner, Assoc.Prof.
Head of Lab for Langerhans Cell Research - DDI: 23016
Christoph H. Tripp, Ph.D.
Senior Scientist- DDI: 28592
Daniela Ortner-Tobider, Ph.D.
Post Doctoral Fellow- DDI: 28592
Nikolaus Romani, Prof. emeritus
Experimental Dermatology
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Medical University of Innsbruck
Department of Dermatology, Venereology & Allergology
Doctoral College: Molecular Cell Biology and Oncology (MCBO)
IMMUTRAIN - an EU Horizon 2020 Network
17th International Workshop on Langerhans Cells, Jerusalem, Israel, Fall / Autumn 2023 !
16th International Symposium on Dendritic Cells, Australia, 2022
Leopold-Franzens University, InnsbruckNCBI - PubMed
European Epidermal Barrier Research Network
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Universitätsklinik für Dermatologie, Venerologie und Allergologie | Anichstrasse 35, A-6020 Innsbruck

Tel.: (+43-512) 504-22971, Fax: (+43-512) 504-22990